Neuroendocrine modulation sustains the C. elegans forward motor state

نویسندگان

  • Maria Lim
  • Jyothsna Chitturi
  • Valeriya Laskova
  • Jun Meng
  • Daniel Findeis
  • Anne Wiekenberg
  • Ben Mulcahy
  • Linjiao Luo
  • Yan Li
  • Yangning Lu
  • Wesley Hung
  • Yixin Qu
  • Chiyip Ho
  • Douglas Holmyard
  • Ni Ji
  • Rebecca D McWhirter
  • Aravinthan Dt Samuel
  • David M Miller
  • Ralf Schnabel
  • John A Calarco
  • Mei Zhen
چکیده

Neuromodulators shape neural circuit dynamics. Combining electron microscopy, genetics, transcriptome profiling, calcium imaging, and optogenetics, we discovered a peptidergic neuron that modulates C. elegans motor circuit dynamics. The Six/SO-family homeobox transcription factor UNC-39 governs lineage-specific neurogenesis to give rise to a neuron RID. RID bears the anatomic hallmarks of a specialized endocrine neuron: it harbors near-exclusive dense core vesicles that cluster periodically along the axon, and expresses multiple neuropeptides, including the FMRF-amide-related FLP-14. RID activity increases during forward movement. Ablating RID reduces the sustainability of forward movement, a phenotype partially recapitulated by removing FLP-14. Optogenetic depolarization of RID prolongs forward movement, an effect reduced in the absence of FLP-14. Together, these results establish the role of a neuroendocrine cell RID in sustaining a specific behavioral state in C. elegans.

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عنوان ژورنال:

دوره 6  شماره 

صفحات  -

تاریخ انتشار 2016